About
Highly accomplished Ph.D. Research Scholar with extensive expertise in molecular biology, phytochemistry, and microbiology, specializing in natural product pharmacology. Proven ability to conduct advanced phytochemical analyses, evaluate bioactive potentials, and investigate complex molecular mechanisms, contributing to significant findings in antibacterial and quorum-sensing inhibition research. Adept at utilizing advanced chromatographic techniques, in silico studies, and statistical analysis to drive impactful scientific discoveries and publications.
Work
University of Kalyani
|Ph.D. Research Scholar
Kalyani, West Bengal, India
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Summary
Led comprehensive phytochemical analyses and bioactivity assessments of Plumbago zeylanica L., investigating its antibacterial and quorum-sensing inhibitory potential to advance natural product pharmacology.
Highlights
Led comprehensive morphological studies and precise phytochemical screening of *Plumbago zeylanica L.*, identifying plumbagin-rich components and quantifying phenolics, flavonoids, and tannins using standard protocols.
Designed and executed robust antibacterial assays against multiple human pathogenic strains (e.g., *E. coli, S. aureus, V. cholerae*), demonstrating broad-spectrum activity and performing antioxidant assays to evaluate extract efficacy.
Utilized advanced chromatographic techniques, including GC-MS, TLC, and HPLC, for meticulous profiling and quantification of plumbagin and other bioactive compounds in plant extracts.
Investigated quorum-sensing inhibitory activity of plumbagin through biochemical and molecular assays, successfully suppressing biofilm formation, violacein biosynthesis, EPS production, and bacterial motility in *Chromobacterium violaceum* ATCC 12472.
Employed in silico docking and simulation to confirm anti-quorum sensing potential of plumbagin at the molecular level by targeting the CviR protein, validated findings using statistical tools (SPSS, GraphPad, Origin, R).
Authored and co-authored multiple research articles and book chapters, effectively documenting and interpreting complex experimental results for publication and presentation in peer-reviewed journals.
Education
University of Burdwan
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M.Sc.
Botany
Grade: 74% (1st Division)
Courses
Cytogenetics
Molecular Biology
IPTG-mediated induction
Expression of Arabidopsis thaliana MYB4 protein
Katwa College, University of Burdwan
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B.Sc. (Hons.)
Botany
Grade: 67.82% (1st Division)
Courses
Botany
Zoology
Chemistry
Katwa Ramkrishna Vidyapith
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Higher Secondary
Science
Grade: 68.72% (1st division)
Katwa Kashiram Das Institution
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Madhyamik
Grade: 73%
Awards
UGC-CSIR NET Fellowship for Ph.D. Research
Awarded By
University Grants Commission (UGC), Government of India
Awarded for qualifying the National Eligibility Test (NET) with All India Rank (AIR-69), jointly conducted by CSIR-UGC, supporting Ph.D. research.
Swami Vivekananda Merit-cum-Means Scholarship
Awarded By
Government of West Bengal
Received for academic excellence and financial need during M.Sc. studies at the University of Burdwan.
Swami Vivekananda Merit-cum-Means Scholarship
Awarded By
Government of West Bengal
Received for academic excellence and financial need during M.Sc. studies at the University of Burdwan.
Publications
Mental healing with herbal medicine: Phototherapeutic approaches to neurological and psychological disorders.
Published by
International Journal of Pharmacy and Pharmaceutical Science
Summary
Review article discussing phototherapeutic approaches using herbal medicine for neurological and psychological disorders.
The anti-quorum sensing and biofilm inhibitory potential of Piper betle L. leaf extract and prediction of the roles of the potent phytocompounds.
Published by
Microbial Pathogenesis
Summary
Research article on the anti-quorum sensing and biofilm inhibitory potential of Piper betle L. leaf extract and phytocompound prediction.
Morphological determinants and phytochemical variations in Plumbago zeylanica l. across four districts of West Bengal.
Published by
Journal of Plant Developmental Sciences
Summary
Research article detailing morphological characteristics and phytochemical diversity of Plumbago zeylanica across different regions.
Distinct antibacterial and antioxidative properties of Couroupita guianensis Aubl. Plant parts and their relation with polyphenolic contents.
Published by
Research Journal of Biotechnology
Summary
Research article exploring the antibacterial and antioxidative properties of Couroupita guianensis and its polyphenolic content.
Languages
English
Skills
Research & Analytical Techniques
Phytochemical Analysis, Quantitative Analysis, Antibacterial Assays, Antioxidant Assays, Quorum-Sensing Inhibition, Biofilm Formation, Violacein Biosynthesis, EPS Production, Bacterial Motility, Gene Expression Analysis, Molecular Assays, Protein Expression Monitoring, SDS-PAGE, Bacterial Transformation, Culture Handling, Experimental Design, Methodology Development.
Molecular Biology & Genetics
PCR, DNA Extraction, RNA Extraction, Gel Electrophoresis, Gene Regulation, Cytogenetics, Molecular Biology.
Chromatography & Spectroscopy
Gas Chromatography Mass Spectrometry (GC-MS), Thin Layer Chromatography (TLC), High-Performance Liquid Chromatography (HPLC).
Microscopy
Confocal Laser Scanning Microscopy (CLSM), Scanning Electron Microscopy (SEM), Light Microscopy, Biofilm Visualization, Cellular Morphology, Microbial Interactions.
Bioinformatics & Computational Tools
Molecular Docking (AutoDock, Discovery), Protein-Ligand Interaction Analysis (FTMap, DeepSite), Sequence Alignment (NCBI, BLAST), Structure Prediction (SWISS-MODEL), QS-Related Gene Analysis, Public Genomic Databases, In Silico Studies, Simulation.
Data Analysis & Statistics
Microsoft Excel, GraphPad, Origin, SPSS, R, Past, Statistical Modeling, Comparative Analysis, Data Validation, Data Interpretation.
Plant Science & Ecology
Plant Physiology, Plant Biochemistry, Natural Product Pharmacology, Phytochemistry, Plant Sampling, Herbarium Techniques, Basic GIS Application, Morphological Studies, Host-Microbe Interaction.
Projects
M.Sc. Dissertation Project: Determination of optimal conditions for IPTG-mediated induction and expression of Arabidopsis thaliana MYB4 protein in E. coli cells.
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Summary
Investigated and optimized parameters for efficient expression of recombinant AtMYB4 protein in *E. coli* BL21(DE3) cells, focusing on concentration, temperature, and incubation time.